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None, Conflict of Interest: Stamp's modified Ziehl—Neelsen staining for Brucella: Beware of the first impressions.
Indian J Med Microbiol ; Due to protean clinical manifestations, correct diagnosis of disease relies largely on laboratory evidence such as antibody detection or culture. Stamp's modified cold Ziehl—Neelsen staining was performed on these blood culture broths using 0. Bacterial colonies were screened by Gram's staining, oxidase test and Christensen's urease test.
Isolates were further confirmed by Vitek 2 Compact system and multiplex polymerase chain reaction PCR targeting bcsp31 gene of Brucella for the simultaneous identification of genus Brucella bpBrucella abortus bp and Brucella melitensis bp.
All the 50 isolates were oxidase positive, but only 45 did hydrolyse urea majorly within 15 min. Isolates showing both oxidase and urease positivity were confirmed as B. Colonies of Brucella were faintly visible after 18 h of incubation while B.
However, all the isolates of Brucella and Brevundimonas were morphologically indistinguishable both in Gram's stain and S-ZN stain.
While an early and rapid detection of Brucella in cultures is important to initiate appropriate antimicrobial therapy, culture manipulations increase the chance of laboratory-acquired brucellosis in under-equipped laboratories of developing nations.
S-ZN staining method serves as a safe and economical method for presumptive identification of Brucella from blood cultures in resource-poor settings.
Brucella is not truly acid fast, but the organisms retain certain dyes, including basic fuchsin, in the presence of dilute acids or alkalies, and this has been used as a basis of differential staining methods.
Brucella organisms are stained pink against a blue background.
Available reports demonstrated that organisms of enzootic abortion Chlamydia and Q fever Coxiella burnetii are also stained pink within tissue cells and are very difficult to differentiate from Brucella organisms. We report for the first time that B.
Adding to the complexity that like Brucella, it appears as small pink-coloured coccobacilli against a blue background when stained by S-ZN staining method. This can result in B. These findings alert microbiologists to use additional tests before declaring pink coccobacilli in S-ZN stained smear as Brucella.
Conflicts of interest There are no conflicts of interest.ICMR JRF Entrance Examination INFORMATION BROCHURE INDIAN COUNCIL OF MEDICAL RESEARCH RAMALINGASWAMI BHAWAN, ANSARI NAGAR, POST BOX , NEW DELHI Organising Institute: Postgraduate Institute of Medical Education & Research Chandigarh The Financial Aid Office at Notre Dame de Namur University offers a complete array of financial and support services which are designed to help you with the education-related expenses involved in obtaining your educational goal.
• Indian Council of Medical Research Financial Assistance Grant to MD/MS/DM/MCH Thesis Programme - Indian Council of Medical Research, New Delhi, • Congress Travel Award by Melvin Yahr International Parkinson’s Disease Foundation and Association of Parkinsonism and Related Disorders - XIX World Congress on Parkinson’s Disease and.
The first author acknowledges the receipt of financial assistance to MD thesis from Indian Council of Medical Research (ICMR), New Delhi, for pursuing this Vol - 4, Issue - 3, Apr-Jul ISSN - Pharma Science Monitor 21 Apr Indian Council of Medical Research (ICMR).
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Dr. Ravneet Singh Grover is a Pulmonary & Critical Care Specialist from Amritsar in Punjab. His special areas of expertise are Bronchoscopy, Thoracoscopy, Critical Care Medicine, Sleep Medicine and Allergy & Immunology. MD Thesis selected by Indian Council of Medical Research (ICMR) under ICMR financial assistance programme. Article. Management of HRD programs, viz. Junior research Fellowship (JRF), Post Doctoral Fellowship (PDF), MD Thesis, International Travel Grant Support, etc. ii. Helping and assisting the Head of . Projects and Guidelines for Operation of Extramural Projects Indian Council of Medical Research V. Ramalingaswami Bhawan, Ansari Nagar, lausannecongress2018.com No. New Delhi ± 2 Tel.: , , GRAM: SCIENTIFIC , FAX: approved for financial assistance. ii. I/We agree to submit within one .
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